Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Effect of alcohol extract of Zataria multiflora (Boiss), Satureja bachtiarica (Bunge) and Zaravschanica membranacea (Boiss) on immuno-hematologic factors in rats

Hamed Soleyman Dehkordi¹, Mohsen Jafarian Dehkordi², Milad Rezamand Chaleshtori³, Faham Khamesipour¹ , Simbarashe Katsande⁴

¹Young Researchers and Elite Club, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran; ²Department of Pathology, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran; ³Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran; ⁴Para-Clinical Department of Veterinary Studies, Faculty of Veterinary Studies, University of Zimbabwe, Harare, Zimbabwe.

For correspondence:- Faham Khamesipour Email: F.Khamesipour@iaushk.ac.ir Tel:+989134132858

Received: 15 May 2015 Accepted: 4 October 2015 Published: 29 November 2015

Citation: Dehkordi HS, Dehkordi MJ, Chaleshtori MR, Khamesipour F, Katsande S. Effect of alcohol extract of Zataria multiflora (Boiss), Satureja bachtiarica (Bunge) and Zaravschanica membranacea (Boiss) on immuno-hematologic factors in rats. Trop J Pharm Res 2015; 14(11):1999-2004 doi: 10.4314/tjpr.v14i10.7

© 2015 The authors.
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Abstract

Purpose: To determine the effect alcohol extract of Zataria multiflora, Satureja bachtiarica and Zaravschanica membranacea on immunohematologic factors in Wistar rats.

Methods: Wistar rats were randomly allocated to seven treatment groups which consisted of control group with water and feed only (1); 200 mg. kg^-1 Z. membranacea (2); 400 mg. kg^-1 Z. membranacea (3); 200 mg. kg^-1 S. bachtiarica (4); 400 mg. kg^-1 S. bachtiarica (5); 200 mg. kg^-1 Z. multiflora (6) and 400 mg. kg^-1 Z. multiflora (7). Erythrocyte counts (RBC), packed cell volumes (PCV), haemoglobin (Hb) concentration, mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), Translocation and Assembly Module (Tam) protein, IgM and albumin were measured after 29 days.

Results: Z. membranacea at 200 mg kg^-1 showed the highest level of Tam-protein content (p < 0.05). Z. multiflora boiss at 400 mg kg^-1 produced higher levels of immunoglobulin M (IgM) compared to S. bachtiarica and Z. multiflora (p < 0.05). Both Z. membranacea and S. bachtiarica at 200 mg. kg^-1 caused a significant increase in albumin levels in the rats (p < 0.05). Z. multiflora at 400 mg. kg^-1 had the highest effect on white blood cells (WBC) while S. bachtiarica produced the highest effect on neutrophils (Nut) (p < 0.05). Z. membranacea and Z. multiflora at 200 mg. kg^-1 showed significantly higher levels of monocytes (Mon) % (p < 0.05). Z. multiflora and S. bachtiarica at 400 mg. kg^-1 showed a significant effect on phagocytosis % (p < 0.05) whilst S. bachtiarica at 400 mg.kg^-1 had a significant effect on phagocytosis number (p < 0.05).

Conclusion: The alcohol extracts of Z. multiflora Z. membranacea and S. bachtiarica extracts are capable of stimulating the immune defense mechanism without causing undesirable effects on hematological parameters.